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Figure 4


Fig. 4. Expression of Hoxd11, Hoxd13 and Hoxa13 in early mouse limb buds. (A-I) Whole-mount in situ hybridization assays for Hoxd11, Hoxd13 and Hoxa13 expression in normal forelimb buds. (A-C) Hoxd11 expression. Its distal domain (asterisk) in the posterior-distal autopod (B,C) marks the future autopod at E12.5 (C). (D-F) Hoxd13 expression. The proximal domain of Hox13 expression (arrowheads) at the 34-35 s stage was gradually lost over time. (G-I) Hoxa13 expression at the stages indicated. E10.5 is equivalent to ~35 s stage. Scale bars: 200 µm. Abbreviations: an, anterior; di, distal; do, dorsal; pr, proximal; po, posterior; ve, ventral; s, somite.





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