DEV014555 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Blatocyst morphology of an aggregate of 31 cells. All parameter values are the same as those used in the calculation presented in Fig. 2. The initial cell aggregate (A) and the simulated blastocyst at t=3000 (B,C) are shown in cross-sectional views of xz- (left column) and yz- (right column) planes. (A) The initial cell aggregate, in which a central (black circle) vertex is replaced by a tetrahedron (t=0), followed by cavity enlargement. (B,C) An aggregate enclosed with the ellipsoidal ZP of long y-axis and long z-axis, respectively.

• Movie 1 -

  Movie 1. Time-lapse recording of the mouse embryo with experimentally enlarged ZP developing from 2-cell to blastocyst. The time-lapse corresponds to the sequential pictures of Fig. 3C.

• Movie 2 -

  Movie 2. An example of the simulation, viewed from cross-sections of the yz-plane. Numbers indicate the time point of the calculation process (t) (see Fig. 4A).

• Movie 3 -

  Movie 3. A stereoscopic view of the blastocyst at t=2000, in which the ZP and some of the TE cells are removed for an internal view. (See Fig. 4C.)