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Fig. 1. Sdmg1 is a transmembrane protein expressed in male embryonic gonads.
(A) RT-PCR showing male-specific expression of Sdmg1 in 13.5
dpc gonads. RT-PCR for Gapdh and RT-PCR without reverse transcriptase
(RT) are shown as controls. (B) Transmembrane helix prediction for
Sdmg1. The DUF300 domain is indicated with a black bar. (C) Topology of
Sdmg1. NIH3T3 cells were transiently transfected with HA-Sdmg1-YFP
(green) and immunostained with anti-HA or anti-YFP antibodies (red). Antibody
accessibility indicates that the N-terminal HA epitope is extracellular and
the C-terminal YFP epitope is intracellular. Scale bar: 10 µm.
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