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Figure 3


Fig. 3. Expression of Sdmg1 during gametogenesis. (A) Sdmg1 is expressed in the testis cords from 15.5 dpc to 10 dpp, and in ovarian follicles from 5 dpp. Sdmg1 immunostaining is shown in green, DNA in red. (B) In situ hybridisation showing Sdmg1 mRNA expression in Sertoli cells in adult testis (antisense probe, purple precipitate). (C) Immunostaining of adult testis cryosections showing Sdmg1 (green) in Sertoli cell cytoplasm. (D) In situ hybridisation showing Sdmg1 mRNA expression in granulosa cells in follicles in adult ovaries. (E) Immunostaining of adult ovary cryosections showing Sdmg1 (green) in the granulosa cells of follicles. Some Sdmg1 is also present in the oocyte cytoplasm (arrows). (F) Immunostaining for Sdmg1 (green) in germinal vesicle (GV) stage oocytes and pre-implantation embryos. Scale bars 100 µm.





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