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Fig. 7. Perturbing secretion induces male-to-female germ cell sex reversal in
embryonic gonad cultures. (A-R) Urogenital ridges (11.5 dpc) were
transiently treated with or without brefeldin A to block secretion, or were
cultured continuously with retinoic acid or ketoconazole. Urogenital ridges
were cultured for 4 days and analysed by histology (Haematoxylin and Eosin) to
assess germ cell development, and immunostained for anti-Mullerian hormone
(AMH, brown precipitate) to assess Sertoli cell development. Urogenital ridges
were also cultured for 6 days and immunostained for synaptonemal complex
protein 3 (Sycp3). Sycp3 labels the thread-like synaptonemal complex in
meiotic oocytes, and is present as large aggregates in prospermatogonia.
Examples of meiotic oocytes are labelled with arrowheads; examples of
prospermatogonia are labelled with arrows. Scale bars: 10 µm.
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