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Fig. 3. Mispositioning of the kinocilium in Ush1 mutant hair cells.
(A) Basal view of E18.5 cochlear whole-mounts from wild-type,
Ush1c-/- and Cdh23v2J/v2J mice were
stained with an antibody that labels the kinocilia (green), and with
phalloidin to reveal F-actin in stereocilia (red). (B) Schematic of the
method used to measure the angular deviation of the kinocilium with respect to
the PCP axis on SEM images. A P0 wild-type OHC is taken as an example.
(C) Evaluation of the kinocilium position on P0 wild-type and
Ush1 mutant hair cells. For each mouse strain, kinocilia were falsely
labeled orange (using Adobe Photoshop) to facilitate their visualization on
SEM images of control and mutant OHCs. Mean absolute positions
(±s.e.m.) of the kinocilia for the total population of hair cells
(orange) and per hair cell row (blue to purple) are represented on the bar
charts. P-values, which were determined with respect to the positions
of kinocilia in wild-type mice using the Welch's t-test, are
indicated by asterisks (*P
0.05,
**P0.01, ***P0.001). The pie
charts present the percentage of hair cells in which the kinocilium is
mispositioned by 0-14° (white), 15-44° (light-gray), 45-89°
(medium-gray), or 90-180° (dark-gray) from its expected location at the
lateral-most edge of the cell surface. Scale bars: 2 µm in A; 1 µm in
C.
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