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Fig. 7. Mouse Ush1 protein localization in late developing Ush1 mutant
hair bundles. (A) Hair bundles of sensory cells from the cochlear
basal turn of P5 Myo7a4626SB/4626SB,
Cdh23v2J/v2J, Pcdh15av3J/av3J and
Ush1gjs/js mice were stained with the harmonin-H1b
antibody (green) and phalloidin (red). Note again the absence of harmonin-b
labeling in hair bundles of Ush1gjs/js and
Myo7a4626SB/4626SB mice, and the presence of
immunoreactive spots that have increased in number and size since E18.5, in
the cuticular plate of the latter mutant (arrowheads). In
Cdh23v2J/v2J and Pcdh15av3J/av3J mice,
harmonin-b is also mislocated, as it is still detected at stereocilia tips
(arrowheads) instead of near the tip link side attachment point (see wild-type
control in B). (B) Cochleas of P5 wild-type and
Ush1c-/- mice were stained with phalloidin (red) and
antibodies to myosin VIIa (Myo7a-F1), harmonin-b (harmonin-H1b), cadherin 23a
(Cdh23-N1) or protocadherin 15a/b (Pcdh15-cter) (green). The stainings for
myosin VIIa, Cdh23-a and Pcdh15-a/b are the same in
Ush1c-/- and wild-type hair bundles. Scale bars: 2
µm.
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