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Fig. 3. Gene-specific RNAi effects in primary cells. Primary cells were
isolated from G053 Drosophila embryos expressing the SLS-GFP fusion
protein and were treated with dsRNAs targeting lacZ
(A,D), sls (B,E) and Mhc
(C,F). SLS-GFP expression was detected by GFP (A-C, green in
D-F). Muscle structure was revealed by SLS-GFP in green, phalloidin staining
of Actin in red, and DAPI staining of nuclei in blue (D,E,F). Scale bar: 20
µm.
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