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Figure 2


Fig. 2. APC/CFzr/Cdh1 functions during endocycles. (A) Schematic representation of Flipout/Gal4 clones induction in the Drosophila salivary gland. Clones are induced during the first larval instar, after the cells have entered the endocycle (Smith and Orr-Weaver, 1991). The Flipout/Gal4 technique was used to clonally express UAS-ApcRNAi constructs with GFP. (B-D''') salivary gland containing (B-B''' and C-C''') Apc8RNAi or (D-D''') Apc8RNAi; Apc10RNAi Flipout/Gal4 clones stained with (B,C,D, white; B''',C''',D''', green) {alpha}-GFP, (B',D', white; B''', D''', red) {alpha}-Geminin and (C', white; C''', red) {alpha}-Cyclin A antibodies and (B'',C'',D'', white; B''',C''',D''', blue) DAPI. (B',D') Geminin and (C') Cyclin A are ectopically expressed in cells that express the Apc8RNAi or the Apc8RNAi; Apc10RNAi constructs. Expression of Apc8RNAi, as well as the co-expression of Apc8RNAi and Apc10RNAi, results in a decrease in the nuclear size (B'', arrowheads; D'', arrows).





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