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Fig, 6. Orc1 is a target of the APC/C^Fzr/Cdh1 during the endocycle. Drosophila salivary glands containing (A-A'') UAS-Apc10^RNAi; UAS-Apc8^RNAi and (B-B'') UAS-Cyclin E Flip-out clones labeled with (A,B, white; A'',B'', red) ${alpha}$ -Orc1 and (A',B', white; A'',B'', green) ${alpha}$ -GFP antibodies, and (A'',B'', blue) DAPI. Orc1 is ectopically expressed in cells that express the UAS-Apc10^RNAi; UAS-Apc8^RNAi or UAS-Cyclin E (marked by an asterisk) constructs in the salivary gland. The arrow indicates a wild-type cell that expresses high levels of Orc1 protein. (C,D) porc1-Orc1-GFP (C) and porc1-Orc1Oboxmut-GFP (D) salivary glands labeled with (white and green) ${alpha}$ -GFP antibody and (blue) DAPI. Higher levels of Orc1 are observed when the O box is mutated. The pictures shown in C and D were taken using the same settings. Wild-type (E-E'') egg chambers and (F-F'') salivary gland stained with (E,F, white; E'',F'',green) ${alpha}$ -Orc1 and (E',F', white; E'',F'', red) ${alpha}$ -MPM2 antibodies, and (E'',F'', blue) DAPI. There is a correlation between the MPM2 spheres and the Orc1 staining.

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