|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Pbx1 binds the Six2 promoter in vivo. (A) PCR
amplification (39 cycles) of the immunoprecipitated chromatin from E11.5
frontonasal mass and first branchial arch (1st) or from second branchial arch
(2nd) using anti-Pbx1 antibody or normal rabbit IgG. EB, elution buffer. ChIP
was performed on two independent pools of samples, and PCRs were performed in
duplicate on each pool. Results shown are from a representative set.
(B,C) Western blot using anti-Pbx1 on whole extracts of human
293 cells transfected with pCDNA3-Pbx1a (Pbx1a) or pCDNA3-Pbx1b (Pbx1b), or
whole extracts from frontonasal mass (FNM), first branchial arch (1st ba) and
second branchial arch (2nd ba) of E11.5 embryos. (D) Ponceau staining
of the membrane shown in C. Arrowhead, position of Pbx1a; arrow, position of
Pbx1b.
|
