spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Expression of Igf1 and Igfbp5 in wild-type and mutant mouse embryos. (A-F) In situ hybridization on whole-mount E11.5 wild-type (A,C,E), Hoxa2 mutant (B,D) and a2-Six2 transgenic embryos (F) using Igf1 (A,B) and Igfbp5 (C-F) probes. The arrow in A and B indicates the embryonic area where Igf1 is upregulated in the mutant. The arrow in C and D indicates the domain where Igfbp5 expression in the Hoxa2 mutant is lost. In E and F, arrows and arrowheads indicate areas of Igfbp5 expression in the second branchial arch; note the reduced Igfbp5 expression in the second branchial arch of embryos overexpressing Six2. I, first branchial arch; II, second branchial arch. (G) Semi-quantitative RT-PCR on RNA extracted from E10.5 second branchial arches of wild-type (wt) and a2-Six2 (tg) embryos using primers specific for Six2 and Gapdh.





Right arrow Return to article