|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Six2 binds the Six5 binding site identified in the mouse Igfbp5
promoter. (A) Incubation of the wild-type oligonucleotide probe in
the presence of unprogrammed reticulocytes does not result in the formation of
a specific retarded complex. Incubation of the probe with Six2-programmed
reticulocytes gives rise to a retarded complex (arrow), which is competed by
the addition of cold double-stranded wild-type oligonucleotide (wt), but not
by oligonucleotide with a nucleotide substitution in the Six5 binding site
(m). Cold oligonucleotides were added at 100- and 500-fold molar excess.
(B) Incubation of the labeled mutant oligonucleotide does not result in
the formation of a specific complex in the presence of Six2-programmed
reticulocytes. (C) Sequence of wild-type and mutant oligonucleotides.
The nucleotide changed in the mutant oligonucleotide is highlighted in
red.
|
