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Fig. 4. AGL24 directly regulates SOC1. (A) Schematic of the Arabidopsis SOC1 genomic region. Black boxes, exons; white boxes, introns and upstream regions. Bent arrows denote translation start sites and stop codons. Arrowheads indicate the sites containing either single mismatch or perfect match with the consensus binding sequence (CArG box) of MADS-domain proteins. Ten PCR fragments corresponding to the DNA sequences near these CArG boxes were designed for ChIP analysis. (B) ChIP enrichment test by quantitative real-time PCR shows the binding of AGL24-6HA to the region near the number 6 fragment. (C) Schematic of the Pro_SOC1:GUS construct. The native CArG box within the number 6 fragment identified in B was mutated as indicated. (D) GUS staining of Pro_SOC1:GUS plants. Representative GUS staining of 12-day-old transformants containing Pro_SOC1:GUS and its mutated form is shown in the upper panels. Representative lines were crossed with 35S:AGL24, and GUS staining of 10-day-old F1 plants is shown in the lower panels. (E) Distribution of relative GUS staining intensity in the transformants containing Pro_SOC1:GUS and its mutated construct. (F) Distribution of flowering time in T1 transgenic plants carrying the wild-type SOC1 gene and its mutated form in the soc1-2 mutant background.

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