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Figure 5


Fig. 5. Hematopoietic and endothelium precursors are defined by CD41 and CD34 relative expression. (A) Cells expressing CD41 and CD34 were sorted from day-5 EBs grown in serum-free conditions supplemented with Bmp4, followed by activin A, bFGF and VEGF at day 2.5. Each fraction was tested for the presence of hematopoietic progenitors by colony-forming ability in secondary replating assay. (B) May-Grunwald Giemsa staining of CD41+CD34- sorted cells; arrow indicates more-mature cells (40x magnification). (C) Day-5 serum-supplemented EBs derived from wild-type ES cells or ES cells carrying a truncated human (h) CD4 cDNA knocked into the Runx1 locus, were stained for CD41 and hCD4 coexpression. (D) The CD41 and CD34 fractions were tested for their ability to form VSM (vascular smooth muscle) colonies upon secondary replating. Data are presented as the mean number of colonies from three dishes. Error bars represent s.e.m. All data shown are representative of at least three experiments. PS, presort.





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