DEV012807 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Light microscopy of vestigial abscission zones in wild-type and bop1 bop2 cauline leaves. (A-C) Longitudinal series from a wild-type cauline leaf. (A,B) Vesitigial AZs are darkened cell files between the stem and the adaxial surface of the leaf (arrows). (C) Once in the vasculature, the vestigial leaf AZ is not obvious. (D-F) Series through a bop1 bop2 cauline leaf does not show vestigial AZs.

• Supplemental Figure S2 -

  Fig. S2. Floral organ abscission zones (AZs) in ida (Col-0×C24) and bop1 bop2 ida (Col-0×C24) plants. (A,B) Scanning electron micrograph of petal AZs. The fracture plane on the receptacle was observed following removal or natural abscission of petals in ida (A) and bop1 bop2 ida (B) from position 2, 4, 6 and 12. (A) Petal AZs in ida show broken cells at position 2 and rounded AZ cells by positions 4 and 6. However, petal fracture planes revert to show broken cells by position 12. (B) Petal AZs from bop1 bop2 ida always show broken cells. (C) Petal breakstrength of bop1 bop2 ida is similar to bop1 bop2. Error bars represent s.d. Scale bars: 400 µm in A,B.

• Supplemental Figure S3 -

  Fig. S3. Real time quantitative RT-PCR of IDA and HAESA in wild-type (Col-0) and bop1 bop2 abscission zones. Transcripts levels of IDA and HAESA were determined using two biological replicates and were normalised using ACTIN2. Amplification efficiency was assumed to be 2. IDA was expressed at 0.94 and HAESA at 0.95 in bop1 bop2 relative to wild type, which was set at 1.00. Error bars indicate the range.