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Fig. 2. Morphology of floral AZs in wild type (Col-0) and bop1
bop2. (A,B) Scanning electron micrographs of
Arabidopsis petal AZs. The fracture plane on the receptacle (arrow)
was observed following removal or natural abscission of petals in wild-type
(A) and bop1 bop2 (B) flowers at positions 2, 4, 6 and 12. (A)
Wild-type fracture planes demonstrate progression from broken cells (position
2) to rounded AZ cells (position 6) to protective surface cells (position 12).
(B) Fracture planes of bop1 bop2 petals from all positions show
broken cells. (C-J) Toluidine Blue-stained sections from flowers at anthesis.
(C-E) AZ cells at the stamen filament-receptacle (C), petal-receptacle
(D) and sepal-receptacle (E) junctions in wild type (arrows). (F)
Longitudinal section along the adaxial and abaxial faces of wild-type flower.
(G-I) Receptacle-organ boundaries of stamen filaments (G), petals (H)
and sepals (I) of bop1 bop2 lack cells displaying AZ anatomy
(arrows). (J) Abaxial petalloid sepal of bop1 bop2 lacks an
organ-receptacle boundary. f, stamen filament; p, petal; s, sepal; ad,
adaxial; ab, abaxial. Scale bars: 400 µm in A,B; 35 µm in C-E,G-I; 70
µm in F,J.
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