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Fig. 4. Expression of BOP1::GUS. (A-I) Whole mounts of
BOP1::GUS (Col-0) transgenic Arabidopsis plants stained for
GUS activity. (A) Expression is restricted to the base of developing
organs in stage 9 and 10 flowers. (B) Expression at the base of floral
organs and in lateral nectaries of a stage 12 flower (arrow). (C) GUS
activity at the base of floral organs, sepal vasculature, style and pollen of
stage 14 flowers. (D) Newly abscised petal and stamen show expression
in their AZs and filaments. (E) Position 9 siliques show AZ expression
in the receptacle AZ. (F) Young pedicel-stem junctions have an abaxial
GUS signal that spreads around the attachment point in older pedicels
(G). (H) BOP1::GUS bop1 bop2 plant with GUS activity at
the base of the ectopic bract (arrow). (I) Cauline leaves show GUS
activity at their base. (J-N) Sections of stained BOP1::GUS flowers.
(J) Stage 5/6 flower showing GUS activity in stamen primordia
(asterisk) and sepals. (K) Staining of a stage 7/8 flower is restricted
to the base of stalked stamens (asterisk) and outgrowing sepals. (L)
Section through the same stage 7/8 flower showing staining throughout the
petal primordia (asterisk). (M,N) Mature flowers with GUS
activity through the AZ (M) and nectaries (N). Scale bars: 250 µm in
A,B,D-F; 0.5 mm in C; 1 mm in G-I; 35 µm in J-N.
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