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Fig. 7. Nectary morphology and CRC::GUS activity in wild type (Col-0) and bop1 bop2. (A,B) Sepals and petals in Arabidopsis were removed from wild-type (A) and bop1 bop2 (B) position 4 flowers. Lateral nectary protrusions are obvious in wild type (arrow) but not in bop1 bop2. (C) Pair of lateral nectary glands with secretory stomata (arrow) in wild type. (D) Lateral nectary glands absent in bop1 bop2. (E-H) Toluidine Blue-stained sections from position 3 flowers. (E) Wild-type transverse section shows paired lateral nectary glands with distinct epidermal and starch-containing parenchymal regions, a ridge of connecting nectary tissue (arrow) and a medial nectary gland. (F) Corresponding bop1 bop2 cross-section shows two primordia (arrows) in the lateral nectary position. (G) A lateral nectary gland visible in longitudinal section of wild type. (H) Corresponding longitudinal section of bop1 bop2 shows a small outgrowth (arrow) in the lateral nectary position. (I-N) CRC::GUS plants stained for GUS activity. (I-K) CRC::GUS in wild type (Col-0xLer). (I) Expression in the nectary anlagens of a stage 7/8 flower (arrow). (J) Strong nectary gland expression in a mature flower. (K) Position 7 flower with GUS staining nectaries and the ring of connecting nectary tissue. (L-N) CRC::GUS activity in bop1 bop2 (Col-0xLer). (L) Expression in stage 7/8 flowers in the nectary anlagens (arrow). (M) Strong expression in the nectary gland region of a mature flower. (N) GUS staining in position 7 flowers shows strong staining in the lateral nectary gland region and weaker staining in the medial and connecting nectary tissue regions. ln, lateral nectary; ls, lateral stamen; mn, medial nectary; p, petal; s, sepal. Scale bars: 500 µm in C,D; 70 µm in E-H,J,K,M,N; 35 µm in I,M.

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