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Fig. 3. Lhx6 expression can rescue interneuron phenotypes in
transplanted cells from Nkx2.1-/- MGE*.
pLhx6-GFP was electroporated into the MGE-like region of E12.5
Nkx2.1-/- slices, then after 1DIV the transfected regions
were dissociated and transplanted into the cortex of neonatal pups. Shown are
coronal sections through a P30 mouse that had received the transplantation
into the cortical plate at P1. (A-H) Examples of co-labeling for GFP
together with Kv3.1 and parvalbumin (PV; A-D), somatostatin (SST; E,F), and
neuropeptide Y (NPY; G,H). In control experiments with pGFP vector, few cells
expressing these markers are detected after transplantation of
Nkx2.1-/- MGE-like progenitors (see text and
Table 1). (I,J)
Transfected neurons (I, pGFP control; j, pLhx6-GFP) photographed at higher
magnification to reveal dendritic spines. Insets show the boxed regions at
higher magnification. (K) The frequency of heavily spiny neurons is
significantly lower in the Nkx2.1-/- MGE* cells
transfected with Lhx6 than in controls (41.9% versus 24.7%,
n=3, *P<0.03). In addition, those
Nkx2.1-/- cells `rescued' for expression of PV or SST by
Lhx6 are nearly all non- or sparsely spiny. These results suggest
that Lhx6 can act downstream of Nkx2.1 to direct some
aspects of both the neurochemical and morphological fates of MGE-derived
cortical interneurons.
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