|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Nkx2.1 activates the expression of an Lhx6 promoter
reporter. Shown are examples of coronal, telencephalic slices at
E13.5+1DIV that were electroporated with the constructs indicated.
(A-C) Constitutively expressing pCAG-DsRed2 (A, pDsRed2) was introduced
into a wt mouse embryo slice together with a reporter construct that contains
2.1 kb of the Lhx6 promoter region placed 5' to IRES-GFP
(p5'-Lhx6-GFP, B). The merged image in C shows that the reporter
construct is detectable in the ventral, Nkx2.1-expressing region
(arrowheads) and not in the electroporated region of the medial cortex
(arrow). (D-F) In marked contrast to B and C, electroporation of
p5'-Lhx6-GFP into the MGE-like region (MGE*) of this slice
from an Nkx2.1 null results in no reporter expression (E,F).
(G-I) However, the expression of p5'-Lhx6-GFP is rescued in an
Nkx2.1-null slice by the addition of exogenous Nkx2.1 (red
signal in G and I is NKX2.1 immunofluorescence). (J-L) A wild-type
slice electroporated with a mutated reporter construct in which only the
NKX2.1 consensus binding sequence has been deleted
(p5'-
-Lhx6-GFP; see Materials and methods). Minimal expression of
GFP is detected in the MGE with this construct (K-L). n=at least five
experiments for each result. MGE, medial ganglionic eminence; LGE, lateral
ganglionic eminence; Ctx, cerebral cortex. Scale bar: 200 µm in A for
A-L.
|
