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Fig. 1. Generation of a NC-specific deletion in Foxd3. (A) The mouse Foxd3 locus, targeting vector and resulting alleles. Purple box indicates the single coding exon. (B) Southern blot analysis using the 3' probe shown in A with BamHI-digested genomic DNA; 6.5 kb wild-type band and correctly targeted 4 kb band are indicated. (C) Genotyping mice with PCR primers (black arrows in A). PCR product from Foxd3 floxed allele is 220 bp, from wild-type allele 130 bp. (D-K) Foxd3 immunohistochemistry. In a control embryo, NC cells express Foxd3 at 8 dpc in the headfolds (D), at 9.5 dpc in migrating NC (F, black arrow), at 13.5 in cranial nerves (H, white arrow) and in the gut coils (J, white arrow). Foxd3 protein is detected in a few cells in headfolds of a mutant 8 dpc embryo (E, black arrow). By contrast, no Foxd3 expression is detected at 9.5 dpc in the trunk of a Foxd3 mutant embryo (G) or in the cranial nerves (I) or gut coils (K) at 13.5 dpc. Foxd3-expressing ventral interneurons are detected in the neural tube of control and mutant embryos (red arrows in F,G). olf, olfactory epithelium.

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