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Fig. 2. Cell fate specification, timing of differentiation and tissue architecture are unchanged in Wnt7b-null lungs. (A-D') E14.5 immunohistochemistry of heterozygous (A-D) and Wnt7b mutant (A'-D') mouse lungs. Blood vessels appear normal in the mutant, as marked by PECAM (A,A'), as does airway smooth muscle, marked by smooth muscle myosin (B,B'). Proximal Sox2 (C,C') and distal Sox9 (D,D') expression are unchanged in mutants. Dotted lines encircle the epithelium. (E-I') At E18.5, immunohistochemistry of heterozygous (E-I) and Wnt7b mutant (E'-I') lungs demonstrates that blood vessels (E,E'), airway smooth muscle cells (F,F'), Clara cells as marked by CC10 (G,G'), and type 2 cells as marked by surfactant protein C (H,H') are unchanged in mutant lungs. Type 1 cells marked by caveolin 1 (I,I') are decreased in number. (J) Quantification of cell types in E18.5 lungs demonstrates that the proportions of Clara, ciliated and mucous cells are unchanged in the trachea and airways of Wnt7b mutants. In the distal saccules, the proportions of cells positive for SP-C or PECAM are also unchanged. Error bars represent 1 s.d. (K) The number of Sox9-positive cells per lung bud tip at E14.5 is unchanged in Wnt7b mutants.

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