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Figure 2


Fig. 2. The disrupted gene in the dimC- mutant is a GATA transcription factor. (A) Schematic of the site of insertion of the pBSR{Delta}Bam disruption vector in the dimC- mutant. The numbers refer to Dictyostelium database identifiers (www.dictybase.org). DDB0217990 is predicted to encode a ubiquitin ligase and DDB0220470 a GATA family transcription factor. (B,C) Quantitative PCR analysis of DDB0217990 (B) and DDB0220470 (C) gene expression in developing wild-type (grey bars) and dimC- mutant cells (black bars). PCR reactions were performed in triplicate and data averaged. Identical results were obtained with mRNA extracted from two independent developmental time courses.





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