DEV020115 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 (Adobe PDF) -

  Fig. S1. Epigenetics and cell cycle timing. (A) The differences in G2 length between sister pairs and randomly generated pairs of cells. Plotted as a frequency distribution of G2 variation between sister cells, representing the time that cells remain in G2 after their sister has divided. (B) A strong correlation is found between S-phase lengths of successive cell cycles.

• Supplemental Figure S2 (Adobe PDF) -

  Fig. S2. Heterochromatin distribution in germinating spores. Images of cells from germinated spores expressing GFP-HcpB. Cells were fixed 4 hours after induction of spore germination, and co-stained with DAPI to visualise nuclei. The right-hand images show one cell emerging from a spore with a characteristic heterochromatin spot.

• Supplemental Figure S3 (Adobe PDF) -

  Fig. S3. The effects of caffeine and NHEJ mutations on the G2−M checkpoint and S-phase length. (A) The effect of 1 mM caffeine on the recovery of cells from treatment with 20 mU/ml bleomycin. After removal of bleomycin, the number of dividing cells is reduced by caffeine. (B) Wild-type, Ku and DNA-PKcs null cells were treated with the indicated concentrations of bleomycin. Lengths of S-phase in treated cells (cells which divided in bleomycin) were averaged. Bars reflect standard error.