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Fig. 5. Visualising mitosis and DNA replication in multicellular
development. Mitotic cells were identified using RFP-H2B as a marker for
chromatin. These cells were followed for up to 2 hours in the slug stage of
development. GFP-PCNA was tracked to identify S-phase cells. The movie is of
the prespore region of the slug. The first five frames include both RFP-H2B
and GFP-PCNA. The RFP-H2B panels show a cell division in the central cell
(arrows). The continuation of the movie (5:00 onwards), showing GFP-PCNA
alone, allows one daughter cell (arrows) to be followed into S phase.
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