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Fig. 8. Identification of a DNA damage checkpoint in Dictyostelium.
(A) Cells were treated with bleomycin for 3 hours as indicated. The
number of cell divisions occurring per hour was scored in mock-treated cells
and those treated with 5 mU/ml or 20 mU/ml bleomycin. (B) After removal
of bleomycin, cells escape the checkpoint and divide with increased
synchronicity, as shown pictorially in these stills from a movie. Arrows
indicate dividing cells.
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