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Fig. 1. Smt3 role on development and metamorphosis. (A) Cartoon of
the ring gland, a neuroendocrine complex located above the brain, composed of:
the PG cells that produce the ecdysone hormone (blue); the corpus allatum
cells that produce the juvenile hormone (purple); and the corpora cardiaca
(red). The PG is innervated by neurosecretory cells depicted in red (only one
hemisphere has been represented). (B,C) Ring gland staining
showing nuclei (purple) and Smt3 expression (green).
(B',C') Green channels showing Smt3 expression only
are shown in black and white. Boxed regions are magnified in
B'' and C''. Smt3 levels are strongly
reduced in smt3i nuclei (C',C'') compared with wild
type (B',B''), although some residual protein can be
observed in smt3i PG cells (arrows). (D-F) smt3i
larvae do not pupariate but continue growing as larvae, becoming approximately
double the weight at 18-21 days AEL (F). (G,H) The morphology
and the number of teeth (arrows) in mouth hooks indicate that smt3i
larvae reached the third instar and stayed at that stage throughout the rest
of their prolonged larval life.
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