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Figure 5


Fig. 5. The structure of the cell membrane is compromised in smt3i PG cells. (A-D) Transmission electron microscopy images of PG cells from WT (A,C) and smt3i (B,D) larvae. (A,B) Intracellular channels (IC, arrows) are severely reduced in number and size in smt3i larvae (B) compared with WT (A), although no differences were observed in number, size or morphology of the mitochondria (Mi). BL, basal lamina. (C,D) The nuclear lamina is thickened in smt3i (D) compared with WT (C), but the nuclear pores appear not to be affected (arrows). Nu, nucleus; Cy, cytoplasm. Scale bars: 500 nm. (E,F) Single confocal micrographs showing PG cell nuclei stained with DAPI (purple) and anti-HRP antibodies (green). (E',F') Single green channels for HRP staining shown in black and white.





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