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Figure 6


Fig. 6. The lipid content of smt3i PG cells is reduced. (A,B) Confocal micrographs showing nuclei marked with DAPI (blue) and lipid droplets stained with Oil Red O (red) in WT (A) and knockdown (B) larvae. Single red channels are shown in black and white (A',B'), and boxed regions are magnified (A",B"). Most smt3i PG cells contain very few droplets (arrow in B"), except for the `giant cells', which accumulate large drops (arrow in B'). (C,D) Filipin staining shows a reduction in sterol droplets (white dots) in smt3i PG cells (D) compared with the number of droplets in WT (C). Arrows in C indicate cells containing several lipid droplets in WT cells, whereas arrows in D indicate remnant lipid droplets in some cells of the smt3i PG. (E) Graph of the average number of Oil Red O droplets per cell present in a single confocal plane in WT versus smt3i PG cells, reflecting a one-third reduction of droplets.





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