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Figure 1


Fig. 1. The eye-to-wing transformation is specific for Antp and dependent on the YPWM motif. (A) Ectopic wings (W) and legs (L) induced on the head of ey-Gal4; UAS-Nact; UAS-Antp flies. (B) Higher magnification of the ectopic wing (W) in A, showing the marginal bristles of the triple row. (C) Higher magnification of the antenna-to-leg transformation in A. The apical bristles on the tibia indicate second leg identity (2nd L). (D) Head of an ey-Gal4; UAS-Nact; UAS-AntpAAAA fly showing no wing structures. (E) Higher magnification of the antenna-to-leg transformation, showing the tibial apical bristles (L). (F) Head of an ey-Gal4; UAS-Nact; UAS-AntpQ50K fly. No ectopic wings are formed. (G) Higher magnification of the transformed arista with a claw (CL). (H) The head of an ey-Gal4; UAS-Nact; UAS-Ubx fly shows no ectopic wing. (I) Higher magnification of the ectopic leg with a claw (CL). (J) Higher magnification of ectopic bristles induced in the eye part of the head. (K,M,O,Q) Bright-field micrograph of third instar discs and (L,N,P,R) the VG protein distribution visualized by immunostaining of the corresponding disc to its left. Ant, antennal discs; Eye, eye discs. (K-N) VG is expressed in the wing pouch in wild-type wing (K,L) discs but not in the eye-antennal disc (M,N). (O,P) VG is ectopically induced in the eye disc of ey-Gal4; UAS-Nact; UAS-Antp larvae. (Q,R) There is no detectable VG protein induced in eye-antennal disc of ey-Gal4; UAS-Nact; UAS-AntpAAAA larvae.





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