DEV017418 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

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• Supplemental Figure S1 -

  Fig. S1. Identification of NCC using HNK-1 and TuJ1 immunostaining of E5 whole guts and sections. (A,B) HNK-1 (A) and TuJ1 (B) labelling, showing the enteric network within the small intestine. The front of migration is at the proximal limit of the umbilicus in both (arrows). (C-H) Adjacent cryosections immunostained with HNK-1 (C,E,G) and TuJ1 (D,F,H). (G,H) The NCC-derived wave-front. (C-F) 100 µm (E,F) and 500 µm (C,D) behind the wave-front. Arrowheads in G and H indicate the most advanced cells.

• Supplemental Figure S2 -

  Fig. S2. TuJ1 (green) and QCPN (red) immunohistochemistry of E10 1-7 ablated + Q trunk and 1-7 ablated + Q3 whole-mount gut preparations. (A) QCPN-positive quail cells contribute to the vagus nerve, but not to the intrinsic ENS of the stomach. (B) Quail cells are present within both the vagus nerve and ENS ganglia of the stomach. Scale bar: 100 µm.

• Supplemental Figure S3 -

  Fig. S3. TuJ1 (green) and QCPN (red) immunohistochemistry of E10 1-7 ablated, 1-7 ablated + Q1 and 1-7 ablated + Q3 whole-mount gut preparations. (A) TuJ1 staining within the duodenum in 1-7 ablated guts. (B) The NC-derived wave-front terminates in the mid duodenum. (C) The ENS network in the duodenum is composed mainly of quail NCC. (D) The migration wave-front of quail NCC terminates in the duodenal loop and is co-incident with TuJ1 labelling of vagal NC-derived cells. (E) The ENS network within the duodenum is composed mainly of quail NCC. (F) The migration wave-front of quail vagal NCC is at the end of the terminal hindgut. Arrowheads show the sacral NC-derived fibres that have projected into the gut wall from the NoR. Scale bar: 100 µm.