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Fig. 5. TuJ1 (green) and QCPN (red) immunostaining of E10 3-6 ablated + Q3, 3-6
ablated + Q sacral and 3-6 ablated + Q trunk whole-mount gut preparations.
(A) Characteristic ENS network and perivascular projections in the
stomach. (B,C) The duodenum (B) and the umbilicus level (C)
contain extensive interconnected ganglia. (D) The cecal buds and
hindgut are completely colonised by NC-derived cells. Similar immunostaining
was also observed in 3-6 ablated + Q sacral and 3-6 ablated + Q trunk guts.
(E-I) 3-6 ablated + Q3 guts showing extensive colonisation by quail NCC
within (E) the duodenum, (F) small intestine, (G) umbilicus, (H) cecal buds
and (I) hindgut. (J-N) 3-6 ablated + Q sacral gut. The coincidence of
TuJ1 and QCPN within (J) the duodenum and (K) small intestine. Fewer quail NCC
were observed within the TuJ1+ ENS network. Only occasional QCPN+ cells were
present within the umbilicus region (L). No quail cells were present in the
cecal bud region (M) or hindgut (N). (O-S) Immunolabelling of 3-6
ablated + Q trunk guts reveals that there are no quail NCC throughout the
entire gut. Scale bar in E: 100 µm.
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