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Fig. 8. QCPN (red) and BrdU (green) immunolabelling with DAPI staining (blue) of
E4.5 1-7 ablated + Q3 embryos. (A,D) QCPN staining of NCC
that have migrated from the grafted neural tube (not shown) towards the
developing foregut. (B,E) BrdU detects proliferating cells
within the embryo and within the NCC adjacent to the foregut, respectively.
Inset in E shows QCPN and BrdU labelled cells. (C,F) Merged
images of (C) A,B and (F) D,E with DAPI staining. Bracketed region in C shows
the remnants of the neural tube following ablation. Arrowheads (E,F) indicate
proliferating quail NCC. fg, foregut. Scale bars: 200 µm in A; 50 µm in
D.