DEV016576 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The fby mutation disrupts formation of the pineal complex. (A,B) Dorsal and (C,D) frontal views of foxd3:gfp expression in the pineal complex of 4-dpf larvae. (A,C) In WT larvae, the parapineal organ (arrowhead) was a group of cells found to the left of and below the pineal organ. (B,D) In fby mutant larvae, the pineal complex was a single mass of cells, without a discrete parapineal organ. Scale bar: 25 µm.

• Supplemental Figure S2 -

  Fig. S2. Parapineal migration is unaffected following disruption of the planar cell polarity pathway. (A-I) Dorsal views of the epithalamus in 24-hpf embryos (A-F) or 48-hpf larvae (G-I). Expression of otx5 (A-C) and tbx2b (D-F), and placement of the gfi1-positive parapineal cells (G-I), are unaffected in homozygous mutants for the planar cell polarity genes knypek (kny) or trilobite (tri). (J-Q) Lateral views of 24-hpf embryos. The Wnt receptor frz7a (blue) and the Wnt ligand wnt11 (blue) are excluded from the otx5-positive cells (red, arrowhead) of the pineal complex in WT (J-M) and fby mutant (N-Q) embryos. Scale bars: 25 µm in A-I,K,M,O,Q; 100 µm in J,L,N,P.

• Movie 1 -

  Movie 1. Time-lapse movie of foxd3:gfp in the pineal complex of a WT embryo. Stills for Fig. 8 were extracted from this movie. Frames were captured every 15 minutes from 31-48 hours.

• Movie 2 -

  Movie 2. Time-lapse movie of foxd3:gfp in the pineal complex of a fby mutant embryo. Stills for Fig. 8 were extracted from this movie. Frames were captured every 15 minutes from 31-49 hours.

• Movie 3 -

  Movie 3. Time-lapse movie of foxd3:gfp in the pineal complex of a tbx2b morphant embryo. Stills for Fig. 8 were extracted from this movie. Frames were captured every 15 minutes from 31-49 hours.