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Fig. 4. Fewer and misplaced parapineal cells in fby mutants.
(A-D,I,J) Dorsal and (E-H) frontal views of 4-dpf zebrafish larvae. (A)
In the epithalamus, expression of gfi1 was exclusive to the
parapineal cells of WT larvae at 4 dpf, which formed a compact organ.
(B) In fby mutants, fewer gfi1-expressing cells
developed than in WT, and they were found as individual cells in the middle of
the brain. (C,D) The position of gfi1-expressing cells
from ten individual WT (C) or fby (D) larvae as represented by
different colored dots for each sample. The vertical axis divides the left and
right sides of the brain, and the horizontal axis is the posterior border of
the habenular nuclei. (E) Double immunofluorescent labeling of a WT
larva with foxd3:gfp (green) and the antibody Zpr1 (red). Zpr1 labels
red-green double cone cells, which were almost exclusively found in the pineal
but not the parapineal (arrowhead). (F) In fby mutants, Zpr1
labeled cells were found throughout the pineal complex, including cells
ventral to the pineal organ. (G-J) Double labeling with
foxd3:gfp (green) and anti-rhodopsin antibody (red).
Rhodopsin-expressing cells were near the dorsal midline of the pineal organ in
WT (G,I) and (H,J) fby mutant larvae. Scale bars: 25 µm.
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