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Fig. 5. Reduced numbers of parapineal cells are able to migrate to the left side
of the brain. Dorsal views of the epithalamus in
(A,B,D,E) 31-hpf zebrafish embryos or
(C,F,G-L) 55-hpf larvae. Expression of foxd3:gfp
labels parapineal precursor cells (A, yellow arrowhead), which are
subsequently ablated with laser pulses (B). The remaining parapineal cells
(average of 5±2 cells, n=18) migrate to the left side of the
brain, as revealed by foxd3:gfp (C) and gfi1 (G) expression.
In H, the position of gfi1-expressing cells in ten larvae are
overlaid, with different colors representing individual samples. (D-F) As a
control, cells contralateral to the parapineal precurors were ablated (red
arrowhead). The number of parapineal cells (10±1, n=8) and
their migration in these controls (I,J) were identical to those in unablated
controls (K,L; 10±2 cells, n=10). Scale bars: 25 µm.
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