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Fig. 4. DRG axon projections to the spinal cord, skin and muscle in wild-type
and Runx3-/- mice at
P0. (A-C) Representative photomicrographs of the immunoreactivity
of TrkC (A',A''; green), PV (B',B''; brown) and Vglut1
(C',C''; brown) in the spinal cord of wild-type (A'-C')
and Runx3-/- (A''-C'') mice. The spinal cords
shown in A and B were from the same littermates, those in C from different
littermates. The relative signal intensity of the immunoreactivity of TrkC
(A'''), PV (B''') and Vglut1 (C''') in the spinal cords along
the dorsoventral axis in wild-type (white squares) and
Runx3-/- (black squares) mice. In total, six spinal cord
sections from two to three animals of each genotype were analyzed.
TrkC+ afferents project to the dorsal horn (DH), the intermediate
zone (IZ) and the ventral horn (VH) in the wild-type mice
(A',A'''), but not to the intermediate zone and the ventral horn in
Runx3-/- mice (A'',A'''). PV+
afferents project to the ventral horn in the wild-type (B',B'''),
but not in Runx3-/- mice (B'',B''').
Vglut1+ afferents project to the dorsal horn, the intermediate zone
and the ventral horn in the wild type (C',C'''), but not to the
ventral horn in Runx3-/- mice (C'',C''').
(D-G) Double staining of TrkC+ (D',E'; green)
and TrkA+ (D'',E''; red), and of TrkC+
(F',G'; green) and CGRP+ (F'',G''; red) axons
in the skin of wild-type (D,F) and Runx3-/- (E,G) mice
from the same littermates. Arrowheads indicate
TrkC+/TrkA+ or TrkC+/CGRP+ axons
(yellow in D'''-G'''). (H-J) Immunoreactivity of TrkC
(H,I''; green) and PV (I''; red) in the muscle of wild-type mice
(merge in I'''), and of TrkC (J; green) in Runx3-/-
mice. TrkC+ axons project to the muscle in wild-type but not in
Runx3-/- mice. PV immunoreactivity was not observed in the
muscle of Runx3-/- mice (data not shown). Data are shown
as mean ±s.e.m.; *P<0.05,
**P<0.01, ***P<0.001. Scale
bars: 100 µm in A'',H,J; 50 µm in E''',G'''; 20 µm in
I'''.
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