DEV015149 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables S1-2 -

  Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Normal expression pattern of Hoxd11, Hoxd12 and Hoxd13 in Ror2^{W749FLAG/W749FLAG} limbs. (A-F) Hoxd11 (A,B), Hoxd12 (C,D) and Hoxd13 (E,F) gene expression was detected by whole-mount in situ hybridization on dissected forelimbs from wild-type (A,C,E) and Ror2^{W749FLAG/W749FLAG} (B,D,F) E12.5 embryos.

• Supplemental Figure S2 -

  Fig. S2. Normal pattern of Shh and Fgf8 expression in Ror2^{W749FLAG/W749FLAG} embryos. (A-L) Shh (A-F) and Fgf8 (G-L) gene expression was detected by whole-mount in situ hybridization on wild-type (WT) and Ror2^{W749FLAG/W749FLAG} embryos at stages E11.5 (top), E12.5 (center) and E13.5 (bottom).

• Supplemental Figure S3 -

  Fig. S3. Absence of Gdf5 expression in the presumptive distal digital interzone of E14.5 Ror2^{W749FLAG/W749FLAG} limbs. (A,B) Serial sections of Ror2^{W749FLAG/W749FLAG} (A) and wild-type (B) forelimbs hybridized with a Gdf5 probe. The location of the distal (2/3) and proximal (1/2) interzones is traced in one of the digits across the series (red and gray circles, respectively). The metacarpo-phalangeal interzone (m/1) is also indicated (green circles).