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Fig. 7. Anlagen growth and chondrocyte proliferation are unaffected in Ror2^{W749FLAG/W749FLAG} embryos. (A,B) Forelimb sections from Ror2^+/W749FLAG (HET, A) and Ror2^{W749FLAG/W749FLAG} (KI, B) E13.5 embryos stained with HAB reveal decreased cell numbers in the cartilagenous condensations of the mutant limbs. Stylopod (s), zeugopod (z) and autopod (a) are indicated. (C-F) High magnification of E13.5 ulna sections stained with HAB (C,E) or HGF (D,F), showing normal cell density and intensity of matrix staining. (G,H) Forelimb sections from Ror2^+/W749FLAG (G) and Ror2^{W749FLAG/W749FLAG} (H) E15.5 embryos stained with HAB and HGF. No overt histological alterations are observed in cell density or matrix staining, but the Ror2^{W749FLAG/W749FLAG} bones are shorter, and have a smaller region of hypertrophy and primary spongiosa. (I,J) Sections of E18.5 humeri from three individual Ror2^+/W749FLAG (I) and Ror2^{W749FLAG/W749FLAG} (J) embryos stained with an anti-BrdU antibody and counterstained with AB. Ror2^{W749FLAG/W749FLAG} humeri have a reduced length and shorter epiphyses (epiphysis range is indicated with horizontal lines). No difference was observed in the density or distribution of BrdU-positive cells. (K-N) BrdU incorporation analysis in E12.5 digital rays (K,L) and proliferative zone of postnatal P5 femurs (M,N, zone delineated) from Ror2^+/W749FLAG (HET) and Ror2^{W749FLAG/W749FLAG} (KI) littermate mice. (K,L) Proliferative rates were assayed in E12.5 rays by measuring the number of BrdU-positive nuclei (red) relative to the total number of nuclei (blue) in the condensation. No significant difference was observed between the genotypes (12.2±0.04% versus 12.5±0.05% in the controls, P=0.069). (M,N) BrdU incorporation analysis in postnatal P5 femurs showed no significant difference between genotypes (383±41.9 versus 373±60.5 cells/mm² in the controls, P=0.90). (O,P) High magnification of the growth plates from the sections shown in M and N, respectively, revealing a normal architecture of the columnar chondrocytes.

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