spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Female-specific patterns of Fgf9/Wnt4 signaling states lead to the defective testis induction caused by delayed Sry expression. (A) Whole-mount in situ hybridization analyses showing Fgf9 and Wnt4 expression in Sry-induced (HS+) and non-induced (HS-) XX Tg gonads at 13 and 18 ts (18-hour culture). The gonadal area is indicated by a dashed line. (B) Real-time RT-PCR analysis showing Fgf9 and Wnt4 transcript levels in the Sry-induced and non-induced XX Tg explants initiated at 18 ts (18-hour culture). The transcript levels in the wild-type XY (black bar) and XX (white bar) explants (HS+) of the same littermates at the same stage are also shown. Vertical axis represents Fgf9 or Wnt4 expression level relative Gapdh. The data represent mean values ±s.e.m. (n=4). (C-E) Time-course immunohistochemical analyses with anti-SOX9 (C,D) and anti-3βHSD (E) antibodies, showing effects of exogenous FGF9 (100 ng/ml) and sFRP2 (1.5 µg/ml) on the initiation (9-hour culture) and maintenance (24-hour and 72-hour culture) of Sertoli cells (C,D) and subsequent Leydig cell differentiation (72-hour culture; E) in XX Tg explants Sry-induced at 18-19 ts. (D) Quantification of the relative number per gonadal area of SOX9-positive cells in the immunostained sections (C) of these XX Tg explants cultured in the presence (black circle) or absence (white circle) of FGF9 and sFRP2 (mean values of cell number ±s.e.m.; n=4). The value in the XY wild-type explants of the same littermates (18-19 ts; 72-hour culture) is set as 100% (18.3±1.5x103 cell number per mm2). Asterisks indicate a significant difference (**P<0.01) as compared with the data from the FGF9/sFRP2-treated XX Tg explants initiated at 18-19 ts. The relative numbers of SOX9-positive cells in the XX Tg explants Sry-induced at 12-13 ts are also plotted in D (white square). (E) The addition of FGF9 and sFRP2 together appears to induce an increase of 3βHSD-positive cell number in the Sry-induced XX Tg explants (18-19 ts) at 72 hours in culture, as compared with those of the Sry-induced explants non-treated (None) or treated with FGF9 alone (+FGF9). Scale bars: 100 µm.





Right arrow Return to article