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Figure 6


Fig. 6. Delayed Sry expression can induce testis development in XX Wnt4+/- genital ridges. (A,B) The genital ridges were isolated from the XX Tg Wnt4+/+ (wild type) or Wnt4+/- embryos at 18 ts. The left genital ridges were treated with HS (HS+), while the right ones were used as non-treated control (HS-). After culture for 72 hours, sagittal serial sections of these XX Tg explants were comparatively analyzed by anti-SOX9 (A) and anti-laminin (B) staining (brown staining). Both maintenance of SOX9 expression and testis cord formation are properly induced in the XX Wnt4+/- explant Sry-induced at 18 ts (right upper panels in A,B). In the non-treated (HS-) Wnt4+/- explant from the same embryo, neither SOX9 expression nor cord formation is detectable (right lower panels in A,B), which is similar to non-treated and Sry-induced XX Tg explants of the Wnt4+/+ wild-type littermate. Scale bars: 100 µm.





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