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Fig. 1. Wnt7b is essential for the development of the medullary
component of the mouse kidney. (A-C) In situ hybridization analysis
of Wnt7b expression in the developing mouse kidney. Prior to ureteric
bud outgrowth, Wnt7b mRNA was expressed at low levels in the
mesonephric/Wolffian duct (WD) epithelium (A). After ureteric bud invasion
into the metanephric mesenchyme, Wnt7b expression was restricted to
the ureteric trunk epithelium (B) and its derivatives, the collecting duct
epithelium and ureter of the kidney (C). Expression was not observed at the
ureteric tips (boundary demarcated by dashed lines in B). Scale bars: 100
µm in A,B; 400 µm in C. (D-M) Hematoxylin and Eosin staining of
kidney sections from wild-type littermates (D,F,H,J,L) and Wnt7b
mutants (E,G,I,K,M) as indicated. The renal medullary compartment (bracketed)
was first evident at E15.5 in wild-type embryos, but was absent from mutants
at this and all later stages. The renal pelvis appeared normal (F and G,
insets). Arrows in I and K point to renal corpuscles adjacent to the pelvic
space. Arrows in panels H and J point to renal corpuscles that lie above the
renal medulla. The P10 ureteric epithelium-specific Wnt7b mutants (M)
exhibited hydroureter and hydronephrosis. Scale bars: 200 µm.
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