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Figure 2


Fig. 2. Lymph node anlagen are present in the absence of lymphatic vasculature in Prox1-/- mouse embryos. Combined staining for MAdCAM1 (green), CD4 (red) and CD45 (blue) indicates that most LN anlagen are present in E14.5 Prox1-/- embryos. Shown are (A-C) mesenteric LNs, (D-F) renal LNs and (G-I) axillary LNs in wild-type, Prox1+/- and Prox1-/- embryos. Arrows indicate MAdCAM1+ cells that encapsulate hematopoietic cells (G) or disorganized clusters of hematopoietic and MAdCAM1+ cells (I). Scale bars: 75 µm in A-I.





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