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Fig. 3. Lymph node anlagen develop normally in Prox1-heterozygous and
Prox1-null mouse embryos. To determine whether PROX1 deficiency
affects LN development in a dose-dependent manner, we compared the LN anlagen
of E14.5 wild-type, Prox1+/- and
Prox1-/- embryos. (A-C) Sections were stained for
CD45 (blue), CD4 (green) and IL7R
(red) to identify clusters of LTi
cells, which form the LN anlagen. (D-I) Adjacent sections were stained
for (D-F) VEGFR1 (green), MECA32 (red) and VEGFR2 (blue) to detect endothelial
cells, and for PROX1 (G,H, green), β-galactosidase (β-gal) (I,
green), podoplanin (G-I, red) and MAdCAM1 (G-I, blue) to detect lymphatic
endothelium and stromal cells within the LN anlagen. LN anlagen of wild-type
and Prox1+/- embryos appeared indistinguishable, although
the organization of the lymphatic epithelium and blood endothelium within the
LN anlagen was disorganized in Prox1-/- embryos. Arrows in
D and E indicate blood vessels, and arrowheads indicate small blood vessels.
Arrows in G and H indicate the lining of the lymphatic endothelium. Scale
bars: 75 µm in A-I.
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