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Fig. 3. Branch dynamics during Nrg1-induced chemotaxis. (A-A'')
Images of a dsRed-electroporated slice perfused with a micropipette
containing recombinant EGF domain from Nrg1 (13 nM) and Alexa 488 (green
channel). To induce drastic changes in direction, cortical interneurons (red
channel) migrating through the LGE were confronted with the micropipette at an
angle that is perpendicular to their normal trajectory. (B-B')
Schematic representation of the trajectory change followed by the cell shown
in C. White arrow indicates the micropipette. (C) Representative
time-lapse sequences of a migrating cell that developed drastic trajectory
changes in response to the chemoattractant. The cell generates a new leading
process toward the pipette immediately before changing its trajectory
(t=1:35). The angle generated before the most significant change in
direction is the largest made by the neuron during this sequence (C). The cell
chose the branch oriented towards the chemoattractant to continue migration.
The gradient is also visualized in red owing to laser cross-contamination.
(C'-C'') Drawings illustrate the morphology of the
cell shown in C. Diagrams in C'' depict the movement of this cell. New
branches are shown in green; chosen branch is tipped with a red arrowhead. The
numbers indicate the angle formed by the branches. Scale bars: 100 µm in
A-A'',B,B'; 25 µm in C,C'.
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