DEV023366 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Minor population of the BrdU-positive cells. (A) Percentage of the BrdU-positive and REPO-positive or -negative cells after pulse-chase labelling. Flies labelled immediately after a 1-day BrdU treatment (0-1d BrdU), and after a 9-day chase period with normal food following a 1-day BrdU treatment (0-1d BrdU-10d). The number above each graph indicates the sample number, n. The percentage of BrdU-positive REPO-negative cells increases during the chase period. (B) A BrdU-positive cell that expresses neither REPO nor ELAV (arrowhead). Dorsal is up. an, antennal nerve; al, antennal lobe. Scale bar: 10 µm.

• Supplemental Figure S2 -

  Fig. S2. Expression pattern of the glial GAL4 driver strain, NP577. UAS-cytoplasmic GFP and UAS-nuclear-localized lacZ (nlacZ) were expressed with NP577. Expression of nlacZ and REPO overlapped, indicating that GAL4 is expressed only in the glial cells. Glial processes, labelled with GFP, form an extensive network that surrounds neural cell bodies in the cortex. Glia within and close to the nerves and neuropiles are also labelled, but those near the brain surface (arrowhead) do not express GAL4 (not labelled with GFP or nlacZ). Thus, NP577 expresses GAL4 in a specific subset of glial cells. Frontal view of the brain. Dorsal is up. an, antennal nerve; al, antennal lobe. Scale bar: 10 µm.