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Fig. 2. Glial division around the antennal nerve after eclosion.
(A,B) An adult fly brain of the wild-type (CS) strain after 10
days of BrdU treatment. Projection of confocal optical sections of the frontal
half of the brain (A) and high-magnification single section of the right
hemisphere around the antennal nerve (B). BrdU-positive cells were found
predominantly around the antennal nerve (arrowheads). (C) Percentage of
the antennal nerves associated with BrdU-positive cells. The colour coding
indicates the percentage of antennal nerves with the indicated numbers of
labelled cells. The sample number (n) is indicated above each bar.
(D) Pulse-chase labelling experiment. Blue and orange bars show the
percentage of antennal nerves with the indicated numbers of BrdU-positive
cells. (E) Percentage of antennal nerves with lacZ-positive
cells induced by MARCM. -, flies without heat shock; +, flies with heat shock
to induce MARCM clones. **, P<0.01 (Fisher's exact
test). (F) A MARCM-labelled cell (expressing β-galactosidase,
arrowhead) also incorporated BrdU. Flies were heat shocked 1 day after
eclosion and fixed 4 days later. Frontal optical section around an antennal
nerve. A merged image (left) and single-channel images (insets) are shown.
Scale bars: 50 µm in A; 10 µm in B,F.
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