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Fig. 3. Glial cell division induced by neural PCD. (A) The percentage
of cells that are REPO-positive or -negative among the BrdU-positive cells
observed around the antennal nerve after a 3-hour BrdU treatment (0-3h BrdU).
The sample number (n) is indicated above the bar. (B,C)
Frontal optical section around an antennal nerve. Dorsal is up. A merged image
(left) and single channel images (insets) are shown. (B) A BrdU-positive cell
expressed the glial marker REPO, but not the neural marker ELAV (arrowhead).
(C) Morphology of a BrdU-positive REPO-positive glial cell visualised by MARCM
(expressing GFP, arrowhead). Flies were heat shocked 1 day after eclosion and
fixed 4 days later. (D,E) Specimens after 10 days of BrdU
treatment. Projection of confocal optical sections around the antennal nerves
(left), and a single optical section of the ovary (right). (D) Wild-type (CS)
flies. BrdU-positive cells were observed both in the brain (arrowheads) and
ovary. (E) The elav-p35 strain, in which neuronal PCD was inhibited
by continuous ectopic expression of p35 in neurons. BrdU incorporation in the
brain was suppressed specifically. Scale bars: 10 µm.
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