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Figure 2


Fig. 2. BMP and Wnt signals are required for the specification of neural crest cells. (A-C) Consecutive sections showing expression of molecular markers in chick explants cultured for 28 hours. (A) Stage 2 B explants (n>30) generated Snail2+ and HNK-1+ cells, but no, or only a few Sox1+, Sox2+ or Ker+ cells. (B) Stage 2 B explants cultured in the presence of Noggin (n=26) generated Sox1+ and Sox2+ cells, but no Snail2+, HNK-1+ or Ker+ cells. (C) Stage 2 B explants cultured in the presence of mFrz8CRD (n>30) generated Sox1+ and Sox2+ cells, but no Snail2+, HNK-1+ or Ker+ cells. Data are represented as mean ± s.e.m. Scale bar: 100 µm. (D,E) Relative Dlx5 and Sp5 mRNA levels measured by quantitative real-time PCR. mRNA expression of the BMP target gene Dlx5 (D) and of the Wnt target gene Sp5 (E) is inhibited in stage 2 B explants cultured in the presence of Noggin (D) or mFrz8CRD (E). Bars represent mean ± s.e.m. of four (D) and five (E) independent experiments.





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